NOTE: This is a flexible term position dependent on project funding. Current funding is expected for at least 2 years.

The Bioscience and Biotechnology Division within the Physical and Life Sciences Directorate at LLNL is seeking to fill a position for a dedicated and hardworking Biosciences Technician with experience in neurobiology, cellular biology and/or molecular biology. In this position, you will provide technical support for establishing and evaluating 2D and 3D primary cultures of the nervous system and related animal research. In addition, you will support the preparation and processing of samples for multiomic analyses. Your role will involve working on defined research projects, where you will be expected to demonstrate initiative, resourcefulness, and a keen attention to detail within a team of scientists. This position is in the Human Health Sciences Group in the Biosciences and Biotechnology Division.

You will:

• Follow protocols for isolating and establishing primary human and rodent neuronal and glia cultures.
• Maintain cell cultures and perform routine cell-based assays (e.g., electrophysiology, optical microscopy, immunostaining, preparation of samples other offline assays).
• Prepare and process samples for multiomic analysis, and perform transcriptional sequencing experiments (e.g. bulk RNA sequencing, single cell RNA sequencing, spatial transcriptomics)
• Provide solutions to problems of moderate complexity that require creative use of established and/or innovative methods.
• Collaborate with colleagues for experimental design, assay performance, data acquisition and analysis.
• Perform technical laboratory procedures, where attention to detail, effective troubleshooting skills and good judgment are required.
• Maintain an accurate and detailed laboratory notebook and maintain complete records of experiments.
• Contribute to basic lab maintenance (restocking, waste removal, organization etc.)
• Participate in preparing for annual safety inspections and maintaining a safe laboratory environment.
• Maintain a clean and safe laboratory work area; perform daily maintenance, clean up, and workspace organization within assigned work areas.
• Perform other duties as assigned.

Additional duties at the SES.2 level:

• Analyze current process procedures and provide feedback for the optimization and improvement of workflow.
• Perform quality control and maintenance of laboratory equipment.
• Provide occasional training on laboratory procedures to other employees.
• Offer feedback, identify areas of improvement and efficiency on laboratory protocols.
• Attend and present technical status reports at meetings.